The agreement involves co-operation in research, promotion, knowledge transfer, and legislation and bunker infrastructure.
It follows on from the LNG Master Plan of the Rhine-Main-Danube corridor in which all parties are involved. The aim of the plan is to put LNG to full scale use as a fuel for inland shipping in the area.
Keen to take advantage of the new offering, the Danser Group, supported by the LNG Master Plan, has begun refitting the engines of its Eiger-Nordwand tug boat.
To support the construction of the required LNG infrastructure in the region, the European Union is providing a €40m subsidy.
Rotterdam Port Authority says this co-operation agreement ties in with its aim to see the market for LNG as a fuel develop to its full potential, and to open an LNG hub in Rotterdam by the end of 2015.
The port already has a terminal dedicated to the storage and handling of LNG. Argonon and Greenstream are the first inland vessels powered by LNG which call at Rotterdam, after the regulations were amended last year to allow inland vessel to bunker.